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  • Title: High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis"cattle type" and "sheep type".
    Author: Douarre PE, Cashman W, Buckley J, Coffey A, O'Mahony JM.
    Journal: J Microbiol Methods; 2012 Jan; 88(1):172-4. PubMed ID: 22033498.
    Abstract:
    In this study, we describe a novel HRM-PCR assay that clearly differentiates the two main types of Mycobacterium avium subsp. paratuberculosis (cattle and sheep) based on the polymorphic variation of a previously described tandem repeat. This modern genotyping technique has several advantages over alternative methods, including cost, ease of use and rapidity.
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