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  • Title: The optical trapping dumbbell assay for nonprocessive motors or motors that turn around filaments.
    Author: Spudich JA, Rice SE, Rock RS, Purcell TJ, Warrick HM.
    Journal: Cold Spring Harb Protoc; 2011 Nov 01; 2011(11):1372-4. PubMed ID: 22046050.
    Abstract:
    In vitro motility assays enabled the analysis of coupling between ATP hydrolysis and movement of myosin along actin filaments or kinesin along microtubules. Single-molecule assays using laser trapping have been used to obtain more detailed information about kinesins, myosins, and processive DNA enzymes. The combination of in vitro motility assays with laser-trap measurements has revealed detailed dynamic structural changes associated with the ATPase cycle. This protocol describes the preparation of biotin-actin filaments and coverslips coated with polystyrene beads. These are then used in optical trapping dumbbell assays to study interactions between motors and filaments.
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