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  • Title: Natural plant genetic engineer Agrobacterium rhizogenes: role of T-DNA in plant secondary metabolism.
    Author: Chandra S.
    Journal: Biotechnol Lett; 2012 Mar; 34(3):407-15. PubMed ID: 22048847.
    Abstract:
    Agrobacterium rhizogenes is a natural plant genetic engineer. It is a gram-negative soil bacterium that induces hairy root formation. Success has been obtained in exploring the molecular mechanisms of transferred DNA (T-DNA) transfer, interaction with host plant proteins, plant defense signaling and integration to plant genome for successful plant genetic transformation. T-DNA and corresponding expression of rol genes alter morphology and plant host secondary metabolism. During transformation, there is a differential loss of a few T-DNA genes. Loss of a few ORFs drastically affect the growth and morphological patterns of hairy roots, expression pattern of biosynthetic pathway genes and accumulation of specific secondary metabolites.
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