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  • Title: [Study on preparation of salidroside and polysaccharide in Rhodiola crenulata].
    Author: Wang CQ, Chen J, Li CD, Wu B.
    Journal: Zhong Yao Cai; 2011 Jul; 34(7):1122-5. PubMed ID: 22066409.
    Abstract:
    OBJECTIVE: To optimize the preparation process of salidroside and polysaccharide in Rhodiola crenulata. METHODS: Water-extraction and alcohol-precipitation was used. The effect of added water volume, decoction time and frequency on water-extracting process was investigated by L9 (3(4)) orthogonal design using extraction rate, content of salidroside and polysaccharide as the assessment indexes. The effect of liquor strength and ethanol concentration on alcohol-precipitating process was investigated as well, using content of salidroside and polysaccharide as the assessment indexes. RESULTS: The optimum conditions for water-extracting process were as follows: decocting 3 times, and each time adding 8 fold of water and extracting 2 h. For alcohol-precipitating process,it was that liquor strength was 0.4 g/mL, and ethanol concentration in liquor was 70%. CONCLUSION: The optimized process has guidance for the development and comprehensive utilization of this plant.
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