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  • Title: A new assay for endonuclease/methyltransferase activities based on graphene oxide.
    Author: Lee J, Kim YK, Min DH.
    Journal: Anal Chem; 2011 Dec 01; 83(23):8906-12. PubMed ID: 22066684.
    Abstract:
    A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclease-mediated DNA hydrolysis induces the recovery of fluorescence intensity of the dye attached to the end of the double-stranded DNA region. This GO-based method allows real-time measurement and quantitative assay for endonuclease/methyltransferase activities in short time.
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