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  • Title: Rapid microscopy technique for detection of Pneumocystis carinii in fresh clinical specimens.
    Author: Chichino G, Bruno A, Cevini C, Gatti S, Scaglia M.
    Journal: Eur J Clin Microbiol Infect Dis; 1990 Aug; 9(8):601-5. PubMed ID: 2209627.
    Abstract:
    A direct method for detection of Pneumocystis carinii was evaluated in 14 patients with impaired immune function (3 seropositive for HIV, 8 with AIDS and 3 with heart transplants) and signs and symptoms suggestive of Pneumocystis carinii pneumonia. Direct examination by phase-contrast and interference-contrast microscopy of fresh clinical specimens obtained by sputum induction, bronchoalveolar lavage or transbronchial lung biopsy was found to be a simple and rapid method for detection of Pneumocystis carinii, the sensitivity of the method being comparable to that of the classical toluidine blue O and Diff-Quik staining methods. These findings suggest that this direct microscopy technique could be considered for routine clinical application in patients with suspected Pneumocystis carinii pneumonia.
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