These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Comparison of three protein extraction methods for proteomic analysis of Alexandrium tamarense with two-dimensional electrophoresis].
    Author: Yuan Y, Zheng W, Li S, Wang G, Zheng T.
    Journal: Wei Sheng Wu Xue Bao; 2011 Aug; 51(8):1113-8. PubMed ID: 22097777.
    Abstract:
    OBJECTIVE: In order to find the best extraction method for proteins of Alexandrium tamarense for two-dimensional electrophoresis (2-DE) analysis. METHODS: Three methods for extracting proteins from A. tamarense were compared, including trihydroxymethyl aminomethane (Tris-HCl ) buffer extraction, trichloroacetic acid (TCA)/acetone precipitation and lysis buffer extraction. Alga was cultivated in normal f/2 media (control) and supplemented with algicidal substances. Proteins obtained using the best extraction method were separated with 2-DE. Protein-expression differences were identified using matrix-assisted laser desorption/ionization-time of flight ( MALDI-TOF) mass spectrometry (MS). RESULTS: Among the three protein extraction methods, lysis buffer extraction shows the best detection of the number and quality of protein spots with a clear background. Then, the lysis buffer extraction method was successfully applied to profiling protein expression in algicidal substances stress conditions and 14 differential expression proteins were identified using MALDI-TOF/MS. CONCLUSION: Lysis buffer extraction was the most effective protein extraction method for Alexandrium tamarense.
    [Abstract] [Full Text] [Related] [New Search]