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  • Title: Improved sample preparation for CE-LIF analysis of plant N-glycans.
    Author: Nagels B, Santens F, Weterings K, Van Damme EJ, Callewaert N.
    Journal: Electrophoresis; 2011 Dec; 32(24):3482-90. PubMed ID: 22102066.
    Abstract:
    In view of glycomics studies in plants, it is important to have sensitive tools that allow one to analyze and characterize the N-glycans present on plant proteins in different species. Earlier methods combined plant-based sample preparations with CE-LIF N-glycan analysis but suffered from background contaminations, often resulting in non-reproducible results. This publication describes a reproducible and sensitive protocol for the preparation and analysis of plant N-glycans, based on a combination of the 'in-gel release method' and N-glycan analysis on a multicapillary DNA sequencer. Our protocol makes it possible to analyze plant N-glycans starting from low amounts of plant material with highly reproducible results. The developed protocol was validated for different plant species and plant cells.
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