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  • Title: Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate.
    Author: Ortíz C, Larrieux N, Medeiros A, Botti H, Comini M, Buschiazzo A.
    Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun; 2011 Nov 01; 67(Pt 11):1457-61. PubMed ID: 22102256.
    Abstract:
    An N-terminally truncated version of the enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi lacking the first 37 residues was crystallized both in its apo form and in a binary complex with glucose 6-phosphate. The crystals both belonged to space group P2(1) and diffracted to 2.85 and 3.35 Å resolution, respectively. Self-rotation function maps were consistent with point group 222. The structure was solved by molecular replacement, confirming a tetrameric quaternary structure.
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