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  • Title: Usefulness of two methods of isoelectric focusing for the erythrocyte acid phosphatase phenotypes determination in human bloodstains.
    Author: Pawlowski R, Raszeja S, Komocka G, Kuczyński S.
    Journal: Forensic Sci Int; 1990; 46(1-2):51-4. PubMed ID: 2210544.
    Abstract:
    The authors tried to compare the usefulness of the isoelectric focusing of EAP in bloodstains on 0.2 mm polyacrylamide gel with their method of determination of the enzyme on 1 mm polyacrylamide gel. Both methods turned out to be useful but better results were obtained on 0.2 mm gel. Isoelectric focusing on the ultra-thin gel is more sensitive; it gives clear enzyme strips, takes less time (30 min) and demands about half the amount of material.
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