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  • Title: Use of stroma for the detection of blood-group antibodies by ELISA.
    Author: Giannitsis DJ, Flierl D, Schuler G, Häcker-Shahin B.
    Journal: Infusionstherapie; 1990 Aug; 17(4):217-9. PubMed ID: 2210864.
    Abstract:
    A micro enzyme-linked immunosorbent assay (ELISA), using lyophilized stroma as carrier of red blood cell antigens, which stay stable longer than usual, using intact erythrocytes, was developed for determination of blood-group antibodies in the AB0, Kell and Lewis-systems. Stroma being fixed on microtiter plates was incubated with antisera and peroxidase-conjugated anti-human globulin. The transformation of the substrate added was determined photometrically. A binding of antibodies to the stroma could be demonstrated up to an antibody dilution of 1:1024 for the ABO-system, of 1:512 for the Kell-system and of 1:64 for the Lewis-system. By standardization of this method the quantitative determination of antibodies becomes possible without being restricted by the limited stability of intact erythrocytes.
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