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  • Title: The high resolution G- and R-banding pattern in chromosomes of river buffalo (Bubalus bubalis L.).
    Author: Iannuzzi L, Di Meo GP, Perucatti A, Ferrara L.
    Journal: Hereditas; 1990; 112(3):209-15. PubMed ID: 2211179.
    Abstract:
    High resolution G- and R-banding patterns in chromosomes of river buffalo (Bubalus bubalis L.) were obtained by using early (G-bands) and late (R-bands) BrdU-incorporation in synchronized cell cultures. To better characterize the river buffalo chromosomes, GTG-, GBG-, and RBG-techniques were used. The total number of bands achieved were 490 (207 G-positive, 207 R-positive, 45 variable, and 31 centromeric regions). Only one common G- and R-banding nomenclature was reported. The number, position and intensity of G bands were highly similar by the structural GTG and the replicating GBG-techniques. However, the replicating G- and R-bands appeared to be more distinct and reproducible than the structural G-bands. Some changes in chromosome nomenclature (chromosomes 1p, 2p, 5p, and 21) were made when referred to the cattle homologues.
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