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  • Title: The presence of both the signal sequence and a region of mature LamB protein is required for the interaction of LamB with the export factor SecB.
    Author: Altman E, Emr SD, Kumamoto CA.
    Journal: J Biol Chem; 1990 Oct 25; 265(30):18154-60. PubMed ID: 2211691.
    Abstract:
    In the accompanying paper (Altman, E., Bankaitis, V.A., and Emr, S.D. (1990) J. Biol. Chem. 265, 18148-18153) a putative SecB binding site was identified in the mature LamB protein. The export of wild-type LamB was unperturbed when this region was removed, however, suggesting the presence of a second site of interaction between SecB and LamB. In this paper we show that the interference caused by export-defective LamB proteins is influenced by the amount of signal sequence that is present. If a large portion of the signal sequence is deleted then the interference levels are significantly reduced. This result suggests that a region of the signal sequence contributes to the interaction of SecB with the LamB protein. Using anti-SecB affinity chromatography, we demonstrated directly that the association of SecB protein with precursor LamB is dependent on the presence of both the LamB signal sequence and the interfering region which maps to amino acids 320-380 of mature LamB. Although the interfering region is not necessary for the export of wild-type LamB under normal conditions, when the signal sequence is mutationally altered the interfering region is required to promote the efficient export of LamB protein. Also, deletion of the interfering region eliminates the ability of wild-type LamB precursor to be maintained in an export competent conformation in vivo. Collectively, our results indicate that efficient export of the LamB protein is achieved by an interaction with SecB that involves both the LamB signal sequence and the interfering region in mature LamB.
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