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  • Title: pH-based cation exchange chromatography in the capture and elution of monoclonal antibodies.
    Author: Ng PK, Snyder MA.
    Journal: J Sep Sci; 2012 Jan; 35(1):29-35. PubMed ID: 22125292.
    Abstract:
    Cation exchange chromatography separates adsorbed proteins by controlling the salt concentration or the mobile phase pH. This study examines the pH-based method for binding and elution of monoclonal antibodies (MAbs). Five different clones with isoelectric points from 6 to 9 were evaluated. We performed our studies using a new chromatography resin (Nuvia™ S), which has high binding capacity. A three-column process incorporating Nuvia S as a capture step was also demonstrated for the purification of MAb from tissue culture fluid. Chromatography performance of Nuvia S was demonstrated in a 50-cycle study.
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