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  • Title: Detection of cystathionine ketimine in bovine cerebellum.
    Author: Ricci G, Vesci L, Matarese RM, Antonucci A, Maggio A, Pecci L, Cavallini D.
    Journal: J Neurochem; 1990 Nov; 55(5):1599-602. PubMed ID: 2213011.
    Abstract:
    A new sulfur-containing cyclic imino acid, cystathionine ketimine, has been detected in bovine cerebellum by gas chromatography, gas chromatography-mass spectrometry, and high pressure liquid chromatography procedures. Gas chromatography and gas-mass analyses are based on derivatization of endogenous cystathionine ketimine with diazomethane after a simple enrichment procedure. The high pressure liquid chromatography procedure takes advantage of the selective absorbance at 380 nm of the phenyl isothiocyanate-ketimine interaction product. The concentration of this new sulfur imino acid found in a pool of four bovine cerebella is approximately 0.5 nmol/g.
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