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  • Title: Preparation of samples for single-worm tracking.
    Author: Yemini E, Kerr RA, Schafer WR.
    Journal: Cold Spring Harb Protoc; 2011 Dec 01; 2011(12):1475-9. PubMed ID: 22135667.
    Abstract:
    Neurobiological research in genetically tractable organisms relies heavily on robust assays for behavioral phenotypes. The simple body plan of the nematode Caenorhabditis elegans makes it particularly amenable to the use of automated microscopy and image analysis to describe behavioral patterns quantitatively. This protocol first describes the preparation and use of media for growing and maintaining worms for tracking. The second part of the protocol describes how to prepare a single young adult worm for recording during video analysis. Although the protocol was developed for use in a single-worm tracker, it addresses factors important for the generation of reproducible, standardized images in all systems.
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