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  • Title: Expression, purification, crystallization and preliminary X-ray diffraction studies of the human keratin 4-binding domain of serine-rich repeat protein 1 from Streptococcus agalactiae.
    Author: Sundaresan R, Samen U, Ponnuraj K.
    Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun; 2011 Dec 01; 67(Pt 12):1582-5. PubMed ID: 22139172.
    Abstract:
    Serine-rich repeat protein 1 (Srr-1) is a surface protein from Streptococcus agalactiae. A 17 kDa region of this protein has been identified to bind to human keratin 4 (K4) and is termed the Srr-1 K4-binding domain (Srr-1-K4BD). Recombinant Srr-1-K4BD was overexpressed in Escherichia coli BL21 (DE3) cells. Native and selenomethionine-substituted proteins were prepared using Luria-Bertani (LB) and M9 minimal media, respectively. A two-step purification protocol was carried out to obtain a final homogenous sample of Srr-1-K4BD. Crystals of native Srr-1-K4BD were obtained using PEG 3350 as a precipitant. The crystals diffracted to 3.8 Å resolution using synchrotron radiation and belonged to space group P2(1), with unit-cell parameters a = 47.56, b = 59.48, c = 94.71 Å, β = 93.95°.
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