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  • Title: Proteome profile of zebrafish Danio rerio olfactory bulb based on two-dimensional gel electrophoresis matrix-assisted laser desorption/ionization MS/MS analysis.
    Author: Singh SK, Saxena S, Meena Lakshmi MG, Saxena P, Idris MM.
    Journal: Zebrafish; 2011 Dec; 8(4):183-9. PubMed ID: 22181661.
    Abstract:
    Zebrafish (Danio rerio), is one of the most extensively studied chordate model animal in the field of neuroscience and development biology. Understanding the proteome profile of various tissues and organs of Danio rerio has become increasingly important for inching it as an alternate translational model animal. This study aimed in understanding the proteome profile of D. rerio brain's olfactory bulb exclusively. The proteome pattern of olfactory bulb of D. rerio was determined using the two-dimensional gel electrophoresis approach involving both full range and three various narrow pH range immobilized pH gradient strips, followed by mass spectrometry and tandem mass spectrometry analysis. 221 various proteins were characterized based on matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry analysis as olfactory bulb-specific proteins. The identified proteins represented various pI, mass, functions, and localization. The different proteins acknowledged in this study were found to be associated with diverse metabolic pathways, enzymatic activities, and neurological functions. Glycolysis, gluconeogenesis, and oxidative phosphorylation are the major metabolic pathways found associated with the identified proteins. The identified protein catalogue was also found to be participating in various network pathways such as GABAergic neurophysiological neurotransmission, remodeling of cytoskeleton, neurofilaments, and cell adhesion. This study provides the D. rerio olfactory bulb two-dimensional gel electrophoresis proteome map and the details of 221 olfactory bulb-specific proteins.
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