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  • Title: Comparison of specific radioactivities of human alpha-lactalbumin iodinated by three different methods.
    Author: Thean ET.
    Journal: Anal Biochem; 1990 Aug 01; 188(2):330-4. PubMed ID: 2221382.
    Abstract:
    Radioiodination provides an extremely sensitive method for the detection of low levels of proteins. In the development of a sensitive radioimmunoassay for human alpha-lactalbumin (alpha-LA), the protein was labeled to high specific activity (approaching 2000 Ci/mmol) with lactoperoxidase, chloramine-T, and Iodogen. Despite high specific activities of the labeled protein by each method, there was a considerable difference in their binding affinity with monoclonal anti-human alpha-LA antibodies due to varying degrees of protein damage. Iodination of human alpha-LA with Iodogen resulted in labels of the highest specific activity and immunoreactivity with the monoclonal antibodies used.
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