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  • Title: A protein-polymer hybrid mediated by DNA.
    Author: Averick SE, Paredes E, Grahacharya D, Woodman BF, Miyake-Stoner SJ, Mehl RA, Matyjaszewski K, Das SR.
    Journal: Langmuir; 2012 Jan 31; 28(4):1954-8. PubMed ID: 22224833.
    Abstract:
    Protein-polymer hybrids (PPHs) represent an important and rapidly expanding class of biomaterials. Typically in these hybrids the linkage between the protein and the polymer is covalent. Here we describe a straightforward approach to a noncovalent PPH that is mediated by DNA. Although noncovalent, the DNA-mediated approach affords the highly specific pairing and assembly properties of DNA. To obtain the protein-DNA conjugate for assembly of the PPH, we report here the first direct copper catalyzed azide-alkyne cycloaddition-based protein-DNA conjugation. This significantly simplifies access to protein-DNA conjugates. The protein-DNA conjugate and partner polymer-DNA conjugate are readily assembled through annealing of the cDNA strands to obtain the PPH, the assembly of which was confirmed via dynamic light scattering and fluorescence spectroscopy.
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