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  • Title: Successful vitrification of mouse ovaries using less-concentrated cryoprotectants with Supercool X-1000 supplementation.
    Author: Tan X, Song E, Liu X, Liu G, Cheng H, Wan F.
    Journal: In Vitro Cell Dev Biol Anim; 2012 Feb; 48(2):69-74. PubMed ID: 22234481.
    Abstract:
    The purpose of our study was to investigate the feasibility of using less-concentrated cryoprotectants supplemented with ice blocker Supercool X-1000 to vitrify ovarian tissues. Mouse ovaries were cryopreserved in different concentrations of vitrification solution alone or with Supercool X-1000, and fresh non-frozen ovaries were used as control. The proportions of morphological normality of follicles, normal GCs in follicular fluids and developing to blastocysts were higher in 12.5% ethylene glycol (EG) + 12.5% dimethylsulfoxide (DMSO) with Supercool X-1000 than those of treated in 10% EG + 10% DMSO or 15% EG + 15% DMSO alone or with Supercool X-1000. In conclusion, the inclusion of Supercool X-1000 in less-concentrated vitrification solution was effective to improve the efficiency and efficacy of cryopreservation of ovarian tissues.
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