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  • Title: DNA extraction from formalin-fixed material.
    Author: Campos PF, Gilbert TM.
    Journal: Methods Mol Biol; 2012; 840():81-5. PubMed ID: 22237525.
    Abstract:
    The principal challenges facing PCR-based analyses of DNA extracted from formalin-fixed materials are fragmentation of the DNA and cross-linked protein-DNA complexes. Here, we present an efficient protocol to extract DNA from formalin-fixed or paraffin-embedded tissues (FFPE). In this protocol, protein-DNA cross-links are reversed using heat and alkali treatment, yielding significantly longer fragments and larger amounts of PCR-amplifiable DNA than standard DNA extraction protocols.
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