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  • Title: [Molecular genetic research of a case of Bx subgroup].
    Author: Liu W, Li D, Liu C.
    Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban; 2011 Dec; 36(12):1213-6. PubMed ID: 22246350.
    Abstract:
    Serologic characters of Bx subtype were analyzed with standard Methods , PCR-SSP method was used for its genotype, and DNA sequences of 6 and 7 exons of ABO genes were analyzed by DNA sequencing. Serologically, the anti-B test was no or 1+, with anti-AB (1+ or 2+). The patient's auto antibodies were negative. PCR-SSP showed the patient was Bx02/O2. ABO genetic locus sequencing was 261delG in exon 6 and 297 was GG homozygous. The sample was a combination of blood group B and O2 because 297A> G, 526C> G, 657C> T, 703G> A, 796C> A, 803G> C, 905A> G, 930A> G shown by direct DNA sequencing and 803G> C, 905A> G shown by cloning DNA sequencing. The serological phenotype of the specimen was B and the genetic sequence was a combination of ABO*Bx.02.1.1 and ABO*O21.01.1.1.
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