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  • Title: [Determination of doxepin in whole blood by SPE-LC-MS/MS].
    Author: Gong FJ, Yan SM, Wu ZP, Zhang RS.
    Journal: Fa Yi Xue Za Zhi; 2011 Oct; 27(5):350-2. PubMed ID: 22259862.
    Abstract:
    OBJECTIVE: To develop a method of SPE-LC-MS/MS for the determination of doxepin in whole blood. METHODS: After solid phase extraction, the samples were identified by LC-MS/MS. Positive ion electrospray ionization mode and multiple reaction monitoring (MRM) mode was selected. Amitriptyline was used as internal standard. The m/z of doxepin: 280-->107, 280-->235 and 280-->220. The m/z of amitriptyline: 278-->233. The retaining time of doxepin and amitriptyline were 15.15 and 16.94 min, respectively. RESULTS: The calibration curve was linear among the concentration of doxepin range from 0.005 to 1.00 microg/mL. The linear correlation equation was y = 3.2047x + 0.0339, the correlation coefficient was 0.9996. The detection limit of doxepin was 0.001 microg/mL and average recovery rate was 78.0%-82.9%. The relative standard precision for within-day and between-day were less than 2.55% and 5.90%, respectively. CONCLUSION: The method is effective, simple, reliable and can be used in the determination of doxepin in whole blood.
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