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  • Title: Analysis of antibody responses by commercial western blot assay in horses with alveolar echinococcosis.
    Author: Ueno M, Kuroda N, Yahagi K, Ohtaki T, Kawanaka M.
    Journal: J Vet Med Sci; 2012 Jun; 74(6):813-5. PubMed ID: 22261148.
    Abstract:
    Commercial western blot (WB) assay was used to detect serum antibodies specific to Echinococcus multilocularis in 23 horses in which infection was confirmed by postmortem inspection at a slaughterhouse. Livers contained from 1 to >20 nodular lesions; foci diameter ranged from 1 to 25 mm. Antibody tests of serum from all 23 animals were negative for antigen bands at 7, 16, 18, and 26-28 kDa, which show specificity in the serum of human patients. However, sera from two infected horses with the largest nodules (diameter, 25 mm) showed positive response to one of the 22-kDa and 30-kDa antigen bands. It may be possible to diagnose E. multilocularis infection in horses based on the detection of these bands on commercial WB assay.
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