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  • Title: gamma-Purothionins: amino acid sequence of two polypeptides of a new family of thionins from wheat endosperm.
    Author: Colilla FJ, Rocher A, Mendez E.
    Journal: FEBS Lett; 1990 Sep 17; 270(1-2):191-4. PubMed ID: 2226781.
    Abstract:
    Two homologous sulfur-rich basic polypeptides form wheat endosperm, so-called gamma 1-purothionin and gamma 2-purothionin, are described. Purification involves extraction with volatile solvents and ammonium bicarbonate fractionation followed by reversed-phase high-performance liquid chromatography. The complete primary structure of these two polypeptides has been determined by automatic degradation of the intact, S-carboxymethylated gamma-purothionins and peptides obtained by enzymatic cleavage. gamma 1-Purothionin and gamma 2-purothionnin consist of 47 amino acids with an molecular weight of 5239 and 5151 Da, respectively and 8 cysteines organized in 4 disulfide bridges. They present a high degree of homology among themselves (89% of identity) and are the first two thionin-like polypeptides, so-called gamma-thionins, described from wheat endosperm.
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