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  • Title: Inhibition of thrombin by synthetic hirudin peptides.
    Author: Binnie CG, Erickson BW, Hermans J.
    Journal: FEBS Lett; 1990 Sep 17; 270(1-2):85-9. PubMed ID: 2226793.
    Abstract:
    To investigate the role of different regions of hirudin in the interaction with the proteinase thrombin, segments of hirudin containing 15-51 residues were synthesized. The C-terminal segment 40-65 inhibited the fibrinogen clotting activity of thrombin but not amidolysis of tosyl-Gly-Pro-Arg-p-nitroanilide. Central peptide 15-42 was insoluble at pH 7, but peptide 15-65 inhibited fibrinogen clotting and amidolysis to an equal extent. The N-terminal loop peptide 1-15 had no inhibitory activity and did not affect the potency of peptide 15-65. These data suggest that the central region inhibits catalysis.
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