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  • Title: Importance of the G27-A43 mismatch at the anticodon stem of Escherichia coli tRNA(Thr2).
    Author: Komine Y, Inokuchi H.
    Journal: FEBS Lett; 1990 Oct 15; 272(1-2):55-7. PubMed ID: 2226835.
    Abstract:
    The tRNA(Thr2) isoacceptor of E. coli has a G-A mismatch at positions 27-43. When the anticodon of this tRNA was converted to an amber anticodon (CUA), this tRNA showed suppressor activity in E. coli. Moreover, introduction of the base pair (G-C or U-A) at positions 27-43 of this suppressor tRNA reduced its suppressor activity. These results indicate that the G27-A43 mismatch is necessary for full function of tRNA(Thr2).
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