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  • Title: Development of EST-SSR markers for Elaeocarpus photiniifolia (Elaeocarpaceae), an endemic taxon of the Bonin Islands.
    Author: Sugai K, Setsuko S, Uchiyama K, Murakami N, Kato H, Yoshimaru H.
    Journal: Am J Bot; 2012 Feb; 99(2):e84-7. PubMed ID: 22282110.
    Abstract:
    PREMISE OF THE STUDY: Expressed sequence tag (EST)-derived microsatellite markers were developed for Elaeocarpus photiniifolia, an endemic taxon of the Bonin Islands. METHODS AND RESULTS: Initially, a complementary DNA (cDNA) library was constructed by de novo pyrosequencing of total RNA extracted from a seedling. A total of 267 primer pairs were designed from the library. Of the 48 tested loci, 25 loci were polymorphic among 41 individuals representing the entire geographical range of the species, with the number of alleles per locus and expected heterozygosity ranging from two to 14 and 0.09 to 0.86, respectively. Most loci were transferable to a related species, E. sylvestris. CONCLUSIONS: The developed markers will be useful for evaluating the genetic structure of E. photiniifolia.
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