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  • Title: Determination of trace vitamin C by ion-pair HPLC with UV detection in calcium gluconate and vitamin C compound oral solution.
    Author: Hu L, Li L, Luo Z, Yang J, Liu W.
    Journal: J Chromatogr Sci; 2012 Feb; 50(2):102-7. PubMed ID: 22298758.
    Abstract:
    A sensitive and specific reversed-phase high-performance liquid chromatographic method with ultraviolet detection was developed for the determination of vitamin C, using tetrabutylammonium hydroxide as an ion-pair reagent in a compound oral solution containing 100 mg/mL calcium gluconate and 1.25 mg/mL vitamin C. The aqueous phase contained 0.005 mol/L tetrabutylammonium hydroxide and the mobile phase consisted of a mixture of the aqueous phase-methanol (80:20, v/v, pH 6.0 adjusted by phosphoric acid). The linearity, sensitivity and specificity, accuracy, and stability of the procedure were evaluated. The calibration curves for vitamin C were linear in the range of 10.0-100.0 µg/mL. The percentage coefficient of variation of the quantitative analysis of the vitamin C in the products analysis was within 5%. The method was successfully applied to determine the stability of vitamin C in the compound oral solution. It was found that the vitamin C peak was symmetrical and the column efficiency was high. The method is simple and suitable for stability testing of a low concentration of vitamin C preparation.
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