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  • Title: Methods to quantitate human haptoglobin by complexation with hemoglobin.
    Author: Cerda S, Oh SK.
    Journal: J Immunol Methods; 1990 Nov 06; 134(1):51-9. PubMed ID: 2230149.
    Abstract:
    Native human haptoglobin isolated from normal human plasma by affinity chromatography on chicken hemoglobin -Sepharose was used as standard antigen. A direct sandwich ELISA for haptoglobin was developed, with human hemoglobin as a capturing agent. The peroxidase activity of the complex was measured as a means of detecting functional haptoglobin. The reactivities of monoclonal antibody vs. polyclonal antisera on the haptoglobin-hemoglobin complex were compared. Adopting a monoclonal antibody, clone 21.7, which is directed to the alpha chain of haptoglobin, a specific method to quantitate the native haptoglobin which can complex with hemoglobin has been developed.
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