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  • Title: A filter-based propidium monoazide technique to distinguish live from membrane-compromised microorganisms using quantitative PCR.
    Author: Hellein KN, Kennedy EM, Harwood VJ, Gordon KV, Wang SY, Lepo JE.
    Journal: J Microbiol Methods; 2012 Apr; 89(1):76-8. PubMed ID: 22314021.
    Abstract:
    Propidium monoazide (PMA) was used to differentiate live from membrane-compromised bacteria in PCR methods. We have adapted this technique for use on membrane-filtered water samples and determined its efficacy using qPCR. Independent labs at three institutions replicated these findings.
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