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  • Title: Naturally co-infected boars with both porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.
    Author: Burgara-Estrella A, Montalvo-Corral M, Bolaños A, Ramírez-Mendoza H, Valenzuela O, Hernández J.
    Journal: Transbound Emerg Dis; 2012 Dec; 59(6):532-8. PubMed ID: 22335816.
    Abstract:
    In this study, the humoral response against porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2), the presence of the virus in semen and serum and the genetic characteristics of the virus detected in 15 boars from a commercial farm were analysed. The results showed that 53% of the boars presented anti-PRRSV antibodies and 100% presented anti-PCV2 antibodies. Porcine reproductive and respiratory syndrome virus was detected in 43% of the boars and 73% were positive to PCV2. The complete ORF5 gene of PRRSV of 14 samples and a fragment of the ORF2 gene of PCV2 of 22 samples were sequenced. Porcine reproductive and respiratory syndrome virus analysis revealed <92% identity in viruses from semen and serum of two boars, whereas in the rest of the boars the identity was >97.5%. As for PCV2, two boars presented an identity <95% in serum and semen and the rest had an identity >96%. The results showed that PRRSV- and PCV2-naturally infected boars can be found, and at least two different strains of viruses from semen and serum can be detected.
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