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  • Title: An investigation on the interaction of DNA with hesperetin/apigenin in the presence of CTAB by resonance Rayleigh light scattering technique and its analytical application.
    Author: Bi S, Wang Y, Pang B, Yan L, Wang T.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2012 May; 90():158-64. PubMed ID: 22336048.
    Abstract:
    Two new systems for measuring DNA at nanogram levels by a resonance Rayleigh light scattering (RLS) technique with a common spectrofluorometer were proposed. In the presence of cetyltrimethylammonium bromide (CTAB), the interaction of DNA with hesperetin and apigenin (two effective components of Chinese herbal medicine) could enhance RLS signals with the maximum peak at 363 and 433 nm respectively. The enhanced intensity of RLS was directly proportional to the concentration of DNA in the range of 0.022-4.4 μg mL(-1) for DNA-CTAB-hesperetin system and 0.013-4.4 μg mL(-1) for DNA-CTAB-apigenin system. The detection limit was 2.34 ng mL(-1) and 2.97 ng mL(-1) respectively. Synthetic samples were measured satisfactorily. The recovery of DNA-CTAB-hesperetin system was 97.3-101.9% and that of DNA-CTAB-apigenin system was 101.2-109.5%.
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