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  • Title: Precise H1N1 swine influenza detection using stuffer-free multiplex ligation-dependent probe amplification in conformation-sensitive capillary electrophoresis.
    Author: Chung B, Shin GW, Hwang HS, Chung YJ, Jung SW, Jung GY.
    Journal: Anal Biochem; 2012 May 01; 424(1):54-6. PubMed ID: 22342882.
    Abstract:
    The H1N1 influenza virus has spread worldwide to become pandemic. Here, we developed a new method to discriminate various types of influenza A, including H1N1, using stuffer-free multiplex ligation-dependent probe amplification based on a conformation-sensitive separation method, namely capillary electrophoresis-single-strand conformation polymorphism. Unlike conventional methods, our approach precisely detects five relevant gene markers permitting confirmation of infection.
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