These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: cDNA isolation, expression, and hormonal regulation of yolk protein genes in the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae).
    Author: Chen SL, Lin CP, Lu KH.
    Journal: J Insect Physiol; 2012 Jun; 58(6):763-70. PubMed ID: 22349178.
    Abstract:
    Yolk protein (YP) or vitellogenin (Vg), the main component of yolk, is the key nutrient for embryonic development. YPs, encoded from uncleaved genes existing mainly in cyclorraphan flies, are different from VGs that are present in most non-cyclorraphan dipterans and other insects. In this study, cDNAs of two YPs, namely Bdyp1 and Bdyp2 (GenBank accession Nos. AF368053 and AF368054), were isolated in the oriental fruit fly, Bactrocera dorsalis (Hendel). RT-PCR analysis revealed that Bdyp1 and 2 are expressed in the fat body and ovary during egg development. However, the expression profiles of Bdyp1 and 2 in the fat body are different, indicating that divergent mechanisms might exist in the regulation of these two genes. Twenty-hydroxyecdysone (20E) plays a major role in promoting Bdyp1 expression, yet the expression of Bdyp2 exhibits a greater response to juvenile hormone (JH) in fat body in vitro. Unexpectedly, 20E-induced expression of both Bdyp1 and 2 is suppressed by JH prior to 20E treatment of in vitro fat body; conversely, it is enhanced by the addition of JH following 20E treatment.
    [Abstract] [Full Text] [Related] [New Search]