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  • Title: [Nucleotide specificity of rat liver thiamine pyrophosphokinase].
    Author: Artsukevich IM.
    Journal: Biokhimiia; 1979 Mar; 44(3):543-7. PubMed ID: 223671.
    Abstract:
    The nucleotide specificity of thiamine pyrophosphokinase from rat liver was studied. The enzyme was found to possess a sufficiently wide substrate specificity. Any of the nucleotides can be a donor of the pyrophosphate groups for TDP biosynthesis at two pH optima of the enzyme in the T-kinase reaction under the Mg2++/NTP optimal ratio. The minimal requirement for the substrate structure allowing to predict the position of the split nucleotide phosphoester bond was postulated.
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