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  • Title: [Isoenzyme spectrum and kinetic properties of pyruvate kinase from the liver of thiamine-deficient rats].
    Author: Konovalenko OV, Maglysh SS, Gorbach ZV.
    Journal: Ukr Biokhim Zh (1978); 1990; 62(4):50-5. PubMed ID: 2238154.
    Abstract:
    Thiamine-deficiency in animals induced by everyday subcutaneous administration of oxythiamine in a dose of 4, 40 and 100 mg/kg of weight for 10 days results in a decrease of the total activity of pyruvate kinase in the liver tissue and does not affect the mentioned index in the kidney and heart tissues. It is shown that as a result of the enzyme fractionation in the column with DEAE-cellulose the total activity of pyruvate kinase in the liver tissue of rats with thiamine-deficiency decreases due to L-isoform while the content of M-isoform remains unchanged. Thiamine deficiency does not affect kinetic characteristics of the L-isoform, extracted from the liver and this shows the absence of changes in the degree of phosphorylation of pyruvate kinase L-isoform under these conditions.
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