These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Visualizing single molecules in living Dictyostelium cells using total internal reflection fluorescent microscopy (TIRFM).
    Author: Matsuoka S, Miyanaga Y, Yanagida T, Ueda M.
    Journal: Cold Spring Harb Protoc; 2012 Mar 01; 2012(3):349-51. PubMed ID: 22383650.
    Abstract:
    Environmental changes result in signaling events at cell membranes. To develop the means to understand these events at the molecular level, it is essential to become familiar with the stochastic nature of signaling molecules in living cells. Using total internal reflection fluorescent microscopy (TIRFM), these signaling events can be directly observed at the single-molecule level. This protocol describes the procedure used for objective-type TIRFM to visualize single fluorescent molecules in living Dictyostelium cells, which are highly sensitive to chemoattractant stimulation. This method can also be applied to other cell types.
    [Abstract] [Full Text] [Related] [New Search]