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  • Title: High transformation efficiency of Bacillus subtilis with integrative DNA using glycine betaine as osmoprotectant.
    Author: Meddeb-Mouelhi F, Dulcey C, Beauregard M.
    Journal: Anal Biochem; 2012 May 15; 424(2):127-9. PubMed ID: 22387342.
    Abstract:
    Electroporation is an important approach for genetic engineering experiments allowing for introduction of foreign DNA in a selected host. Here, we describe for the first time the use of glycine betaine as an osmoprotectant for electroporation of gram-positive bacteria Bacillus subtilis. High electroporation efficiency (up to 5×10(5) cfu/μg) was obtained using 7.5% glycine betaine. The new method improved the transformation efficiency of B. subtilis with linear integrative DNA nearly 700-fold compared with existing Bacillus transformation techniques.
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