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  • Title: [DNA extraction from decomposed tissue by double-digest and magnetic beads methods].
    Author: Yang D, Liu C, Liu H.
    Journal: Fa Yi Xue Za Zhi; 2011 Dec; 27(6):441-3. PubMed ID: 22393595.
    Abstract:
    OBJECTIVE: To study the effect of the double-digest and magnetic beads method for DNA extraction from 3 types of decomposed tissues. METHODS: DNA of cartilages, nails and joint capsule in 91 highly decomposed corpses which had not been extracted by common magnetic beads method, were prepared with the double-digest and magnetic beads methods, and quantified with Quantifiler kit, followed by amplification with Sinofiler kit or Minifiler kit. RESULTS: DNA concentration extracted from the 91 highly decomposed cartilages, nails and joint capsule samples was 0-0.225 ng/microL. Sixty-two samples whose DNA concentration were more than 0.020 ng/microL had obtained 9 or more STR loci successfully. The detection rate was 68.13%. CONCLUSION: The successful rate of STR genotyping for the 3 types of decomposed tissues can be significantly improved by the double-digest and magnetic beads methods.
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