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  • Title: A novel method to measure glucose uptake and myosin heavy chain isoform expression of single fibers from rat skeletal muscle.
    Author: Mackrell JG, Cartee GD.
    Journal: Diabetes; 2012 May; 61(5):995-1003. PubMed ID: 22396201.
    Abstract:
    Skeletal muscle includes many individual fibers with diverse phenotypes. A barrier to understanding muscle glucose uptake at the cellular level has been the absence of a method to measure glucose uptake by single fibers from mammalian skeletal muscle. This study's primary objective was to develop a procedure to measure glucose uptake by single fibers from rat skeletal muscle. Rat epitrochlearis muscles were incubated ex vivo with [(3)H]-2-deoxy-d-glucose, with or without insulin or AICAR, before isolation of ~10-30 single fibers from each muscle. Fiber type (myosin heavy chain [MHC] isoform) and glucose uptake were determined for each single fiber. Insulin-stimulated glucose uptake (which was cytochalasin B inhibitable) varied according to MHC isoform expression, with ~2-fold greater values for IIA versus IIB or IIX fibers and ~1.3-fold greater for hybrid (IIB/X) versus IIB fibers. In contrast, AICAR-stimulated glucose uptake was ~1.5-fold greater for IIB versus IIA fibers. A secondary objective was to assess insulin resistance of single fibers from obese versus lean Zucker rats. Genotype differences were observed for insulin-stimulated glucose uptake and inhibitor κB (IκB)-β abundance in single fibers (obese less than lean), with decrements for glucose uptake (44-58%) and IκB-β (25-32%) in each fiber type. This novel method creates a unique opportunity for future research focused on understanding muscle glucose uptake at the cellular level.
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