These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Zwitterionic polymer-based platform with two-layer architecture for ultra low fouling and high protein loading.
    Author: Huang CJ, Li Y, Jiang S.
    Journal: Anal Chem; 2012 Apr 03; 84(7):3440-5. PubMed ID: 22409836.
    Abstract:
    High resistance to nonspecific adsorption typically accompanies loss of binding capacity and vice versa for many surface coatings and applications. In this study, a zwitterionic polycarboxybetaine acrylamide (pCB)-based binding platform with a "two-layer" structure for ultra low fouling and high protein loading properties was developed. The first pCB layer with a high packing density prepared under a water-free condition serves as a protective layer to resist nonspecific adsorption from complex media. The second pCB layer with a low packing density is used to achieve high protein binding capacity. Amounts of tetraethylthiuram disulfide (TED) and water in the reaction were varied to regulate the packing density and chain length of polymers, respectively, for the second pCB layer. The in situ modification of pCB films with antihuman thyroid stimulating hormone (TSH) IgG molecules and the detection of TSH antigens were employed to demonstrate high protein immobilization and high antigen detection capabilities of this "two-layer" structure. Undiluted blood plasma was used to test the nonfouling properties of this platform. Nonspecific and specific interactions were monitored by a surface plasmon resonance sensor. This work demonstrates great promise of this "two-layer" binding platform for the improved performance of biosensors.
    [Abstract] [Full Text] [Related] [New Search]