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  • Title: Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain.
    Author: Verma A, Hirsch DJ, Hanley MR, Thastrup O, Christensen SB, Snyder SH.
    Journal: Biochem Biophys Res Commun; 1990 Oct 30; 172(2):811-6. PubMed ID: 2241971.
    Abstract:
    ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 microM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3.
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