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  • Title: [Degenerate oligonucleotide primed-PCR technology establishment and its sensitivity test analysis].
    Author: Deng JQ, Liu BQ, Cai JF, Li WH, Long R, Hou YP.
    Journal: Fa Yi Xue Za Zhi; 2012 Feb; 28(1):41-3. PubMed ID: 22435337.
    Abstract:
    OBJECTIVE: To establish a whole genome amplification testing system based on degenerate oligonucleotide primed-PCR (DOP-PCR) and to explore its reliability and sensitivity. METHODS: DOP-PCR amplified production was detected by fluorescent labeled multiplex STR amplification and capillary electrophoresis detection system to determine reliability and sensitivity of DOP-PCR system. RESULTS: DOP-PCR system was successfully established and the detection sensitivity reached 5 cells (30 pg) by pretreatment of DOP-PCR and then detection of STR genotyping. CONCLUSION: The system established in this study is reliable and more testing sensitive for forensic trace evidence.
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