These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Generation and partial characterization of monoclonal antibodies against solubilized membrane fraction of human spermatozoa.
    Author: Yang YZ, Lee CY.
    Journal: Proc Chin Acad Med Sci Peking Union Med Coll; 1990; 5(2):64-8. PubMed ID: 2243834.
    Abstract:
    Human sperm membrane antigens extracted by deoxycholate (DOC) were used to immunize BALB/c mice. Hybrid cell lines secreting sperm-specific monoclonal antibodies were generated by cell fusion in a semi-solid medium and screened by indirect immunofluorscent assay using live and methanol-fixed sperm. Out of 850 hybrid clones from cell fusion, 28 were shown to secrete sperm-specific antibodies which reacted with the acrosome, equatorial segment, whole surface plasma membrane or tail of spermatozoa. Finally, seven hybrid cell lines were established and shown to secrete monoclonal antibodies which had no cross-reactivity with any human tissues other than testis and sperm. The majority were also shown to inhibit fertilization of mouse oocytes in vitro and human sperm penetration of zone-free hamster ova. Western blot analysis revealed that some of these antibodies reacted with sperm membrane antigens of distinct molecular size.
    [Abstract] [Full Text] [Related] [New Search]