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  • Title: [Pronase E and P immobilization on aminoorganosilica surface].
    Author: Bogatskiĭ AV, Davidenko TI, Chuenko AV, Ianishpol'skiĭ VV, Tertykh VA.
    Journal: Ukr Biokhim Zh (1978); 1979; 51(4):315-8. PubMed ID: 224541.
    Abstract:
    Immobilization of pronase E and P was performed on sylochrome modified by gamma-aminopropyltrietoxysilane using cyanuric chloride, 2,4--toluylenediisocyanate, glutaric aldehyde and also on sylochrome by means of titanium tetrachloride. The esterase and caséinolytic activities of the immobilized preparations and their stability during storage were determined. The increased thermostability of the immobilized preparation is established. The influence of the enzyme: carrier ratio on the immobilization process and activity of the enzymes was studied. It is shown that application of glutaric aldehyde favours the best retention of the esterase activity (73%), whereas the caseinolytic activity is better retained (31%) when titanium tetrachloride was used.
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