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  • Title: Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad.
    Author: Hartmann EM, Badalamenti JP, Krajmalnik-Brown R, Halden RU.
    Journal: Appl Environ Microbiol; 2012 Jun; 78(12):4493-6. PubMed ID: 22492441.
    Abstract:
    We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenase-encoding genes.
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