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  • Title: Combined GTG-banding and nonradioactive in situ hybridization improves characterization of complex karyotypes.
    Author: Smit VT, Wessels JW, Mollevanger P, Schrier PI, Raap AK, Beverstock GC, Cornelisse CJ.
    Journal: Cytogenet Cell Genet; 1990; 54(1-2):20-3. PubMed ID: 2249470.
    Abstract:
    Nonradioactive in situ hybridization (ISH) using biotinylated centromere probes for chromosomes 1, 6, 7, 10, 16, 17, 18, and the X, respectively, was combined with GTG-banding to study cytogenetic changes in two different ovarian cancer cell lines. ISH was performed after GTG-banding on the same metaphase. The use of a low trypsin concentration (0.01%) in the banding procedure was essential for subsequent ISH. This combined approach allows the detection of subtle chromosomal rearrangements and appears to aid the identification of marker chromosomes.
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