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  • Title: Detection of cells captured with antigens on shear horizontal surface-acoustic-wave sensors.
    Author: Hao HC, Chang HY, Wang TP, Yao DJ.
    Journal: J Lab Autom; 2013 Feb; 18(1):69-76. PubMed ID: 22496249.
    Abstract:
    Techniques to separate cells are widely applied in immunology. The technique to separate a specific antigen on a microfluidic platform involves the use of a shear horizontal surface-acoustic-wave (SH-SAW) sensor. With specific antibodies conjugated onto the surface of the SH-SAW sensors, this technique can serve to identify specific cells in bodily fluids. Jurkat cells, used as a target in this work, provide a model of cells in small abundance (1:1000) for isolation and purification with the ultimate goal of targeting even more dilute cells. T cells were separated from a mixed-cell medium on a chip (Jurkat cells/K562 cells, 1/1000). A novel microchamber was developed to capture cells during the purification, which required a large biosample. Cell detection was demonstrated through the performance of genetic identification on the chip.
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